Eppendorf InjectMan® NI 2 Micromanipulator

A menu-controlled, programmable micromanipulator, the InjectMan NI 21 is especially suitable for microinjection into adherent cells. The electronic connection of the InjectMan NI 2 and the FemtoJet®2 or FemtoJet express ensures a very rapid and safe microinjection. The semiautomatic microinjection allows a coordinated process of manipulation and injection: first, the injection parameter is set on the FemtoJet; second, the injection level is fixed via pressing a button on the InjectMan NI 2. The exact axial injection movement ensures a minimum mortality rate, and its high speed facilitates the penetration of rigid structures.

Semiautomatic microinjection into adherent cells

The electronic coupling of the InjectMan NI 2 with the FemtoJet makes microinjection into adherent cells possible. This semiautomatic sequence of manipulation and injection occurs first by programming the injection parameters on the FemtoJet and then establishing the injection level (Z-limit) by pressing a button on the InjectMan NI 2.

By activating the joystick key, the patented axial injection movement of the capillary and the programmed pressure increase can be simultaneously triggered on the FemtoJet. Following the preset injection time, the pressure decreases in the capillary and is triggered simultaneously with the movement of the capillary back to its starting position. Thus, the capillary tip serves as a “pointer” for the target location in the cell, and the exact axial injection movement secures the highest survival rate.* With the help of this technology, it is now possible to process more than 40 cells per minute simply, efficiently and reproducibly.