 |
FV1000
Olympus FV1000 Confocal Microscope
|
 |
 |
Olympus FluoView Confocal Laser Scanning Microscopes - FV1000
The Olympus FluoView™ FV1000 is a next-generation imaging system designed for high-resolution, confocal observation of both fixed and living cells. The FV1000 offers advances in confocal system performance while providing the speed and sensitivity required for live cell imaging with minimal risk of damage to living specimens.
In addition, the FV1000 offers a revolutionary synchronized laser scanning system called the SIM Scanner. While one laser stimulates, the second laser simultaneously provides high-resolution imaging. This coordination of laser stimulation and imaging makes the FV1000 an ideal choice for FRAP, FLIP and photoactivation.
Specifications |
||
| Laser Source | Visible | Select
from the following lasers to be mounted on laser
combiner: Multi-line Argon Laser (457nm, 488nm, 515nm), total 30mW HeNe-Green Laser (543nm), 1mW, or Krypton Laser (568nm), 10mW, special order HeNe-Red Laser (633nm), 10mW |
| Violet/UV | Select
from the following: 405nm Laser Diode, 6mW 440nm Laser Diode, 0.7mW UV-Argon (351nm), 40mW, special order |
|
| Laser Control | AOTF intensity control and shuttering for each laser line in UV and visible light range. Integrated system control for diode lasers. Both controls provide laser turn-off during retrace period and REX mode scanning. REX (region of excitation) mode permits laser intensity and wavelength selection for each designated region of excitation. Integrated shutters are included for all laser lines. | |
| Laser Feedback Monitor | Included within the excitation light path of the main scan unit. Provides continuous intensity feedback to laser control. Maintains consistent laser power to the sample during time series experiments. | |
| Scan Unit | Standard Configuration | 3
internal PMT detectors for fluorescence detection + 1
external PMT detector for transmitted light, 3 laser
ports (visible, UV, IR) Additional optional light paths available, including: 4th Channel PMT detector unit Fiber port for fluorescence output to fiber optic SIM Scanner |
| Scan Method | 2 galvano mirrors | |
| Pinhole | One
common pinhole for all channels. Pinhole diameter: 50-800µm (50-300µm for spectral system) Pinhole continuously adjustable in 0.5µm increments. |
|
| Scan Modes | Normal,
Clip, Zoom-In, Bi-Directional Fast Scan, Line, Point XY Scan Dimensions: Time, Z, lambda (for spectral system) Pixels: 64x64 - 4096x4096 Aspect ratio: 1:1, 4:3, Arbitrary ratio Rotation: 360-degree Scan Speed (Pixel duration): Normal Mode: 2µs - 5ms, 9 steps, 500-5KHz Bi-Directional: 1KHz, (512x512, 2x zoom) 2KHz, (256x256, 5x zoom) Line Scan Dimension: Time, Z, lambda (for spectral system) Type: straight line, rotation, free line Number of lines: 1 - 32,500 Lines Bi-Directional: 2-4KHz/line Fast XZ: 2msec/line |
|
| Field Number (FN) | 18 | |
| Zoom | 1x
- 50x 0.1x increments |
|
| Z-drive | Motorized
focus through internal stepper motor. Minimum step increment 0.01µm. |
|
| PMT Detection | High-sensitivity,
side-on PMT detectors, selected for high efficiency. Analog accumulation (AAC) and hybrid photon counting (HPCM) modes. Excellent for quantitation and photometric analysis. High S/N for imaging of low intensity samples. |
|
| Filter Selection | Ion deposition filters. 6 filter positions available for each excitation, spectral and emission filter turret. Automated filter selection through software. | |
| Spectral
System (optional) |
Two
detection channels, each equipped with an independent
diffraction grating and slit for spectral separation
and high-speed bandwidth selection. Selectable wavelength range: 1-100nm per channel Wavelength resolution: 2nm Wavelength change speed: 1ms/100nm |
|
| Microscopes | Inverted:
IX81 Upright: BX61/62, BX61WI |
|
| Optical Path | Motorized Selection of Optical Path: LSM / Reflected Light Illuminator / SIM Scanner | |
| Anti-vibration | Air anti-vibration table | |
| Transmitted Light Detection | Built-in transmitted light detector and halogen light source, connected to microscope via fiber cable. Motorized exchange between halogen light illumination and transmitted laser light detection. | |
| External Fluorescence Illumination | External
fluorescence light source, connected to microscope via
fiber cable. Built-in automated shutter. Motorized exchange between LSM and fluorescence illumination. |
|
| PC Control Unit | PC-AT
compatible OS: Windows XP Professional CPU: Pentium 2GHz or higher Memory: 1GB or larger Hard Disk: 80GB or larger Special I/F board (built-in PC) Graphic Board: ATI RADEON 9200 Memory Media: built-in DVD-R/RW Monitor: two (2) 20-inch monitors standard, single monitor configuration optional |
|
| Light Path Options | SIM Scanner | For
photoactivation or photobleaching by second,
independent scan unit. Scanning device: 2 galvanometer mirrors (point scanning), built-in laser shutter. Standard configuration: single laser port for 405nm Laser Diode or 488nm Argon laser. Application software and power supply unit required. |
| 4th
Channel PMT Detector Unit |
Directly couples an independent PMT detector to basic scan unit. Increases detection capability of basic scan unit to 4 internal detectors for fluorescence + 1 external detector for transmitted light. | |
| Fiber Port | Fiber
port for fluorescence output to fiber optic. Equipped with FC connector, compatible fiber core 100-125µm. |
|
| Principal Software Functions | Software | Basic acquisition software, application and analysis software. |
| Image Format | XML
format, JPEG, BMP, TIFF and AVI movie format 8-bit and 16-bit gray scale 24-bit, 32-bit and 48-bit full color |
|
| Image Acquisition | Region
designation: point, line, free line, clip, clip zoom 2-dimension: XY, XZ, XT, and *Xë 3-dimension: XYZ, XYT, XZT, *XYë, *XëT and *XëZ 4-dimension: XYZT, *XëZT and *XYëT Real-time image calculation: Kalman filtering, peak detection Time series controller, protocol processor *Spectral system only |
|
| Image Display | Display:
Single-channel, side-by-side, merge, live tiling, scan
parameters LUT: Individual color setting, pseudo-color Overlays: graphic and text input |
|
| 3D Visualization and Observation | 3D animation, left/right stereo pairs, red/green stereoscopic images and cross sectional display, volume rendering | |
| Fluorescence Separation | Fluorescence
separation through spectroscopy: normal and blind
modes Spectral system only |
|
| Image Processing | Individual filters: average, low-pass, Sobel, Median, Prewitt, 2D Laplacian, edge enhancement, etc. Calculations: inter-image, mathematical and logical, DIC background leveling | |
| Image Analysis | Overview of fluorescence intensity, length, area and perimeter measurement, time-lapse measurement | |
| Statistical Processing | 2D data histogram display, co-localization | |
| Others (options) | Time course software, review station software, XY motorized stage control software | |
| Power Consumption | Microscope:
6Amps Scan Unit: 6.2 Amps Computer: 4.5 Amps Multi-line Argon laser: 100Volts, 10Amps HeNe laser: 0.5 Amps each Krypton laser: 20 Amps |
|
