Olympus FluoView Confocal Laser Scanning Microscopes - FV300

Two high-sensitivity photomultiplier tubes (PMTs) are located directly within the FV300 confocal fluorescence emission light path for high sensitivity detection of the fluorescence signal. A separate, dedicated PMT may be used for the simultaneous detection of high resolution Brightfield or DIC images with which the confocal fluorescence images may be overlaid. Up to three channels of detection may be imaged simultaneously. Images may be scanned in any pixel array size up to 2048 x 2048, with each image digitized in 4096 (12-bit) gray depth to permit observation of fine image detail. The images can be displayed side-by-side or in overlay modes.   A single confocal aperture located directly within the emission light path is comprised of 5 distinct user-selectable sizes. The FluoView FV300 software indicates appropriate aperture selection based on the numerical aperture of the objective in use. Various lasers, including the Argon (488nm), Green Helium Neon (543nm), Yellow Krypton (568nm),

Red Helium Neon (633nm), and Helium Cadmium (442nm) lasers are available to suit most fluorescence applications. Each laser line may be independently regulated through software-controlled neutral density filter wheels or through acousto-optical tunable filter for simultaneous or automated-sequential collection of an multi-channel images. A multi-line Argon laser (458/488/515nm) is available with the acousto-optical tunable filter.

Flexibility in the selection of the microscope base, laser lines and optical filters permits researchers to configure the FV300 system for their specific research needs.

Features and Benefits

Confocal microscopy improves the resolution of conventional fluorescence images by recording   fluorescence generated from the focal plane within the sample, while rejecting all other light coming   from above or below the focal plane. The efficient point-scan/pinhole-detection confocal optics of   the FluoView systems virtually eliminates "out of focus" light to produce high-contrast images with superb resolution.

• The FluoView systems are fully integrated workstations that incorporate user-friendly image acquisition   and image analysis software with high resolution confocal optics that require no user alignment.
• An intuitive, Windows NT-based graphic user interface allows new users to quickly generate images in    various scan modes, such as XY, XZ, XT, XYZ, XYT, and XYZT. Optional image formats, including TIFF   and AVI, permit easy, direct export of FluoView images to additional analysis packages.
• XY scanning is performed with a pair of galvonometric mirrors, yielding a wide scanning range to cover   up to a field number of 20 (FV300)/18 (FV500). The optical zoom (up to 10x magnification) can be   performed by narrowing the scanning range while maintaining the maximum pixel resolution of up   to 2048 x 2048 pixels.
• Fully automated Olympus microscope platforms can be interactively controlled through the FluoView   software, with internal stepper motor Z-resolution of 0.01 micron.
• Time lapse observation is possible on the FluoView with imaging rates of up to 4 frames/sec at   512 x 512, and line scan intervals at 0.5msec per line. Kinetic and ratio metric analysis is available for   quantitation of live cell changes over time. Real time intensity plotting and ratio metric image   display are possible.
• TTL I/O signals can be generated to coordinate experiments timed with external instrumentation.
• Attractive pricing for both the FV300 and FV500 provides confocal options to satisfy both individual   laboratories as well as core facilities.

Optics

The entire range of Olympus UIS optics is compatible with the FluoView Confocal Microscope systems.

Accessories

• Transmitted Light Detector with Brightfield and/or DIC optics
• Observation Tube with Video Port
• Tiempo Time Lapse and Ratiometric Analysis Software