Olympus FluoView Confocal Laser Scanning Microscopes - FV300
Two high-sensitivity photomultiplier tubes (PMTs) are located directly within the FV300 confocal fluorescence emission light path for high sensitivity detection of the fluorescence signal. A separate, dedicated PMT may be used for the simultaneous detection of high resolution Brightfield or DIC images with which the confocal fluorescence images may be overlaid. Up to three channels of detection may be imaged simultaneously. Images may be scanned in any pixel array size up to 2048 x 2048, with each image digitized in 4096 (12-bit) gray depth to permit observation digitized in 4096 (12-bit) gray depth to permit observation of fine image detail.
Specifications |
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Visible light |
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laser source |
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Select from the following laser and to be mounted on laser combiner: |
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Multi Ar laser (458nm, 488nm, 515nm, Total 40mW), |
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Ar laser (488nm,10mW), Kr laser (568nm, 10mW), HeNe (G) laser |
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(543nm,1mW), HeNe (R) laser (633nm,10mW), HeCd (Helium cadmium) |
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Laser (442nm 6mW) |
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| UV laser |
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| IR laser |
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| Laser combiner |
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Each laser light path is equipped with a continuously variable neutral |
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density filter or AOTF All laser lines are combined to apsis along |
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the same fiber optic |
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Scanning method |
Galvanometer mirror scanners (both X and Y) |
| Field number (N.A.) |
20 |
| Pinhole |
5-position pinhole turret |
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Image memory |
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and scanning speed |
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Standard scanning mode: 256 x 256 (0.45s) - 2048 x 2048 (10.835s) |
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(Simultaneous scanning up to 5 channels) Bi-directional high-speed |
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scanning mode: |
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512 x 512 (0.25s) (Simultaneous scanning of up to 2 channels) |
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| Image channel |
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lSelectable from 2-channel (fluorescence) or |
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l 2-channel (fluorescence) + 1-channel (transmitted light) |
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Selection of filters |
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according to staining |
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Manual selection |
| Scanning
modes |
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l 1-dimension: Point scanning |
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l 2-dimension (space): X-Y, rect, X-Z, linear line-Z and free line-Z |
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l 2-dimension (time): X-t, linear line-t and free line-t |
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l 3-dimension (space): X-Y-Z and rect-Z |
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l 3-dimension (time): X-Y-t, X-Z-t and rect-t |
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l 4-dimension: X-Y-Z-t and rect-Z-t |
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12-bit(=4096 grey levels) per channel |
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| Zoom |
1X-10X
(0.5X-step) |
| Z-drive |
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Step motor/Minimum step 10nm (BX61, BX61WI and IX81 combination), |
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25nm (other microscope combination) |
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| x |
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External transmitted |
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light unit |
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Transmitted
light |
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illumination
unit |
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External
halogen light source connected to microscope
via fiber cable |
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Transmitted
light |
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detector |
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External detector unit with built-in photomultiplier Connected to microscope |
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frame
via fiber cable |
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Standard
equipment of FV500-BX61, FV500-BX61WI,
FV300-BX51, |
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FV-300-BX61, FV300-BX61WI |
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| y Fluorescence illumination unit |
Connect
to external mercury light source and microscope via
fiber cable |
| z PC with system control boards |
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IBM PC-AT compatible, OS:
Windows NT4.0, Windows 2000 |
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Memory:
1GB RAM or larger, CPU: Pentium 4 1GHz or higher, |
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Hard disk: 40GB or larger, Special I/F board, Image acquisition: PCI bus, |
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Monitor: Selectable from 21, 19, 17-inch, |
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1280 x 1024,
Full color (16.77million colors) |
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| { |
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Fluoview |
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application software |
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Image acquisition |
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Scanning condition setting: image size, scanning speed, zoom, |
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panning etc., Real-time image calculation: Kalman filtering, |
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peak integration, |
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PAPP (Programmable Acquisition Protocol Processor) |
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| Hardware control |
Laser, scanning unit, microscope |
| Image display |
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Each image display: Single-channel side-by- side, merge, cropping, |
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tiling, series (Z/T) pass and continuous LUT: Individual color setting, |
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pseudo-color Overlay: Lines, text, scale bar, etc |
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| Image processing |
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Individual filter: Average, Low-pass, High-pass, Sobel, Median, Prewitt, |
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2D Laplacian, edge enhancement etc. Calculations: Inter-image, |
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mathematical and logical, DIC back ground leveling |
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| Image analysis |
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Overview of fluorescence intensity within an area, histogram, |
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perimeter measurement for user-assigned area, time-lapse measurement |
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etc. TIEMPO (Time course software) |
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| 3D
visualization |
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3D animation, left / right stereo pairs, |
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red / green stereoscopic images and cross section |
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| Others |
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Graphic-based help, time course software (optional), |
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trigger IN/OUT function (optional), 3D software / LSM viewer (optional) |
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| Power consumption |
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l Microscope (115V 6A/230V 3A), |
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l Scanning unit+PSU (115V 3.5A/230V 2A), |
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l Computer & monitor (115V 4.5A/230V 10A), |
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lAr laser (115V 10A/230V 5A), |
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lMulti Ar laser (115V 10A/230V 5A), Kr laser (230V 20A), |
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lHeNe laser each (115V 0.4A/230V 0.2A), UV laser (230V 30A), |
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lIR laser (115V 1A/230V 0.5A), |
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lHeCd laser (115V 5A/230V 3A) |
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