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- Intuitive slide interface
- Meta data display
- Thumbnail image display
- Open
- Close
- Save
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- Automated microscopes
- CCD cameras
- Fluorescence shutters
- Transmitted light shutters
- Filter wheels, turrets, and monochrometers
- Objective turrets
- x, y stages
- z axis control systems
- Magnification changer / optovar
- Prism turrets
- Port controls
- Lamp intensity
- LCD filters
- Imaging splitter
- Motorized InFocus spherical abberration correction device
- Mosaic™ Digital Diaphragm System for FRAP
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- Controls most scientific-grade digital cameras
- Simultaneous dual-camera capture
- Interactive control of speed, gain, and intensification (when possible)
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- 2D (single and multi-fluorphore)
- 2D timelapse (single and multi-fluorphore)
- 3D (single and multi-fluorophore)
- 4D (3D timelapse, single and multi-fluorophore)
- Multiwell plate capture
- Automated objective, filter, X-Y-Z location detection and capture time
- Image comments and naming
- Interactive "mark event" buttons
- Autofocus
- Adjust z position during 4D capture
- Live region intensity graph display
- View previous timepoints during ongoing capture
- Adjust capture rate during timelapse capture
- Multi-position or "6D" (x, y, z)
- Mid-volume capture
- Mixed transmitted / fluorescence capture
- Store and retrieve capture parameters
- User controlled capture feedback
- Single fluorphore
- Color composite view
- Histogram
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- Image renormalization (alter look-up tables)
- Linear filtering
- User defined
- Gaussian
- Gaussian derivative
- Mean
- Median
- Hot pixel
- FFT bandpass
- Laplacian 2D
- 3D edge detection
- No neighbors deconvolution
- Channel math
- Image math
- Flat field correction (shading correction)
- White balance
- FFT
- Photobleach correction
- Background correction
- Crop
- Rotate
- Interpolated image (3D and 4D)
- Intensity projection image (minimum, maximum, and sum)
- Merge timelapse images
- Average images
- Fluorphore alignment
- Timelapse frequency alignment
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- Interactive montage boundary selection
- Automatic stage movement
- Automatic image alignment
- Manual image adjustment
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- ROI selections
- Laser power control
- Laser pulse rate control
- Hardware control during image capture
- Cell Selector module
- Automated Fragment Retrieval (AFR) module
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- RGB, grayscale, inverse grayscale, pseudocolor, gated pseudocolor, user-defined color
- Display up to 8 fluorophores in a single view
- Tile view (3D, timelapse, 4D)
- Three view (3D, timelapse, 4D)
- Main view (axis selection)
- Channel view (display all fluorophores individually in grayscale plus merged color image)
- Multiple volume rendering modes for 3D and 4D data (MIP, dynamic lighting, fixed lighting)
- QTVR volume rendering
- QuickTime movie generation (3D and timelapse)
- Isosurface rendering
- 2D intensity surface plot
- 3D prism generation
- 3D and 4D data rotation
- Particle tracking path display (timelapse)
- Annotations (timestamps, scale bars, object IDs, notes, look-up tables)
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- Intensity segmentation/thresholding
- Gradient segmentation/thresholding
- Magic tools for local segmentation/thresholding
- Manual mask editing
- Binary operations (AND, OR, NOT)
- Erode
- Dilate
- Skeletonize
- Shrink to fit
- Object splitting
- Object classification based on statistics
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- Area/volume (2D/3D)
- Perimeter/surface area (2D/3D)
- Center of area/volume (2D/3D)
- Major and minor axis length (2D and 3D)
- Center of intensity (2D and 3D)
- Mean adjusted center of intensity (2D and 3D)
- Integrated intensity (2D and 3D)
- Mean, minimum, and maximum intensity (2D and 3D)
- Variance and stadard deviation of intensity (2D and 3D)
- Distance - linear and nonlinear (2D and 3D)
- Speed and path length of tracked objects (2D timelapse)
- Object counting (2D and 3D)
- Center to center or edge to edge inter-object distance calculation (2D and 3D)
- Object overlap (2D and 3D)
- Objects containing other objects (2D and 3D)
- Object classification based on morphometry or intensity statistics
- Colocalization coefficients (Pearson's and Manders')
- Line intensity plot (2D and 3D)
- Angles (2D and 3D)
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- Plug-in architecture for end-user writable features
- Automatic methods for generating and storing statistics
- Advanced clustering techniques
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- TIFF, PICT, raw, QuickTime, FluoView, Zeiss 510LSM, BioRad PIC, and more
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- User definable park channel for spinning disk
- Disk speed control for Yokogawa CSU-22
- Laser power control
- Laser sleep mode control
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